肝细胞肝癌(HCC)是我国恶性肿瘤死亡的主要原因之一，随着靶向药物如索拉非尼、阿帕替尼等的出现，其预后得到一定改善，但5年生存率依然较低。新的治疗方式的发现依赖于对肝癌发生发展相关机制的进一步理解。 长链非编码RNAs(LncRNAs(Long noncoding RNAs))是一种长度超过200个nt的非编码RNA。研究表明LncRNAs参与细胞增殖、凋亡、迁移、自噬、干性维持等多个生理过程。LncRNAs表达和功能的异常通过影响细胞增殖、抗凋亡、肿瘤侵袭转移、肿瘤耐药等生物学行为进而导致多种肿瘤的发生。目前为止，LncRNAs在肝癌中的表达、功能及机制仍不完全明确，需要进一步深入研究。 鉴于LncRNA在细胞活动中的重要作用，对肝癌相关LncRNA的功能及其与肿瘤发生发展的关系的研究，探究其上下游可能的效应分子，明确其可能的信号通路，为肝癌的生物靶向治疗提供新的治疗靶点，可能改善肝癌的预后。本课题拟研究LncRNA HOTAIRM1在肝癌中的表达情况、生物学功能以及可能的机制。

 Hepatocellular carcinoma (HCC) is one of the main causes of malignant tumor death in China. With the emergence of targeted drugs such as sorafenib, its prognosis has been improved, but the 5-year survival rate is still low. The discovery of new treatment modalities relies on a further understanding of the mechanisms involved in the development of liver cancer. Long noncoding RNAs (LncRNAs) are non-coding RNAs over 200 nt in length. Studies have shown that lncRNAs are involved in many physiological processes such as cell proliferation, apoptosis, migration, autophagy, and sternness maintenance. The abnormal expression and function of lncRNAs lead to the occurrence of various tumors by affecting biological behaviors such as cell proliferation, anti-apoptosis, tumor invasion and metastasis, and tumor resistance. So far, the expression, function and mechanism of lncRNAs in liver cancer are still not completely clear, and further research is needed. In view of the important role of LncRNA in cell activity, the relationship between the function of IncRNA related to liver cancer and its relationship with tumorigenesis, explore the possible effector molecules upstream and downstream, and clarify its possible signaling pathway for biotargeted therapy of liver cancer. Providing new therapeutic targets may improve the prognosis of liver cancer. This study is to study the expression, biological function and possible mechanism of IncRNA HOTAIRM1 in liver cancer